UniZig: PIONEERING RESEARCH IN BIOTECHNOLOGY
The University of Zagreb (UniZg), particularly its Faculty of Food Technology and Biotechnology, plays a pivotal role in the INBIOWAL project, contributing its expertise in green chemistry, biocatalysis, and sustainable practices. Renowned as Croatia’s leading institution in food technology, biotechnology, and nutritional sciences, UniZg brings innovation and scientific rigor to addressing the challenges of waste valorization.
Role in INBIOWAL
UniZg leads research in the extraction of bioactive compounds, utilizing Natural Deep Eutectic Solvents (NADES), a revolutionary method that aligns with the principles of green chemistry. This research focuses on isolating high-value phenolic compounds and proteins from by-products of the edible oil industry. The institution also drives studies to ensure scalability, environmental compatibility, and industrial applicability of these methods.
UniZg’s role extends to fostering collaboration and knowledge transfer through workshops, joint experiments, and academic exchanges, with a focus on creating actionable solutions for industries such as food, cosmetics, and pharmaceuticals.
Research Excellence and Achievements
UniZg has an established reputation for excellence in international research collaborations, evident from its involvement in various European projects such as PHOENIX, Strength2Food, and Eurocaroten. These projects highlight the institution’s ability to integrate cutting-edge science with societal needs, particularly in food sustainability, nutritional quality, and the development of environmentally conscious technologies.

NADES Expertise
The University’s research in NADES has set benchmarks for eco-friendly extraction techniques, widely regarded as transformative for the food and cosmetics industries. NADES, known for their low toxicity and high efficiency, provide an alternative to traditional solvents, reducing environmental impact while enhancing the quality and yield of extracted bioactive compounds.

Applied Biocatalysis
The University has advanced the use of enzymes and microorganisms in biocatalytic processes, particularly in stereoselective reductions, enzymatic hydrolysis, and oxidative transformations. These methodologies align with global goals for cleaner production and reduced reliance on chemical-intensive processes.

Valorization of By-products
The UniZg team has demonstrated expertise in transforming agricultural by-products into functional ingredients, such as antioxidants and emulsifiers. Recent studies include the use of NADES for stabilizing biomolecules and isolating active compounds from flaxseed and hempseed press cakes.
International Collaboration
UniZg’s strong track record in international partnerships enhances its contributions to INBIOWAL. Notable collaborations include projects within the PRIMA initiative and Erasmus+, which address regional and global challenges in food systems and biotechnology. UniZg has successfully worked with institutions from Spain, Germany, Italy, and other European countries, developing innovative technologies for food packaging, Mediterranean diet promotion, and bio-waste valorization.
- Highlight: PRIMA Projects: Projects such as FUNTOMP and FLAT BREAD MINE showcase UniZg’s interdisciplinary approach, combining food science with advanced processing technologies to create healthier, sustainable food products.
Academic Exchange and Training
As part of INBIOWAL, UniZg facilitates academic exchanges, providing PhD students and researchers with opportunities to work alongside its esteemed scientists. These exchanges enable participants to gain expertise in NADES-based extractions, biocatalysis, and cytotoxicity testing of bioactive compounds. Such initiatives are integral to building a new generation of scientists skilled in sustainable practices.
UniZg also hosts webinars and workshops on topics like:
- Environmental and economic sustainability of NADES-assisted processes.
- Rational design of NADES for specific applications in life sciences and industry.
Future Directions
Through its leadership in Work Package 4 (Extraction of Bioactive Compounds) and active participation in joint events and publications, UniZg aims to:
- Bridge the gap between academic research and industrial needs.
- Expand the industrial applications of NADES, particularly in creating products for health and wellness sectors.
- Strengthen ties with global partners to drive innovation in biotechnology and sustainability.